Isolation of E.coli O157:H7 Bacteriophages of Livestock Waste of Mazandaran Province (Case Study: A Phage Therapy Approach)

Nima Ghahremani Nejad

Department of biology, Faculty of sciences, Islamic Azad University, Tonekabon branch,Tonekabon,Iran.

DOI : http://dx.doi.org/10.13005/bbra/2386

ABSTRACT: Phage therapy for E.coli O157:H7 in animals is considered as a popular research topic. E.coli O157: H7 is a pathogenic potential. It can cause hemolytic uremic syndrome (HUS). Considering this matter, thepresent research aims at analyzing the isolation of E.coli O157:H7 bacteriophages of livestock waste of Mazandaran province using a phage therapy approach. The separation and culture processes of E.coli O157:H7 from livestock wastewaterhave been analyzed in this research. Also, differential tests were used to identify E.coli O157:H7. First, the Lytic bacteriophages of E.coli O157:H7 were isolated from livestock wastewater. Then, both identification and susceptibility tests for bacteria E.coli O157:H7 were done and phages were isolated using various methods. Finally, phage therapy to animal model was conducted. In this study, I have used two methods including disk diffusion and overlay tests. This test can be used for three strains standards including Enteropathogenic E.coli (EPEC), Enterotoxigenic E. Coli (ETEC), Enteroinvasive E. coli (EIEC), and wild E.coli O157:H7. According to the results, 45 samples had lytic phages vs. four different E. coli strains and only 19 samples had significant effect on E.coli O157: H7.Also, it can be concluded that isolated phages affect bacteria in a significant manner (p<0.05).In the current research, the isolated lytic phages removed the infection of E.coli O157:H7 of five groups of mice. As a result, the lytic bacteriophages have the ability to replace antibiotics.

KEYWORDS: E.coli O157:H7; livestock; Mazandaran; mouse; phage therapy

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