Identification of the Carriers of Genes for Resistance to Wheat Leaf Rust Using Molecular Markers

A. Madenova¹,  A. Kokhmetova², G. Kampitova² , M. Atishova²,  L. Purnhauser³  

¹Kazakh National Agricultural University, Kazakhstan, 050010, Almaty, Abay ave. 8 ²Institute of Plant Biology and Biotechnology,Kazakhstan, 050040, Almaty, Timiryazev Str 45 ³Cereal Research Non-Profit Ltd. Co., Hungary, H-6726, Szeged, Alsókikötő-sor 9

ABSTRACT: Wheat leaf rust is an important disease of wheat, which causes economic damage to the country. Epiphytotic diseases of wheat rust cover the entire continents leading to catastrophic crop failures. In order to control the stability, it is very important to have available molecular genetic markers linked to these symptoms. As a result of the phytopathological evaluation of susceptibility to rust on the background of infectious diseases, we selected a number of samples resistant to Puccinia recondite f. sp. tritici using the following molecular markers: F1.2245/Lr10-6/r2, csLV34, LN2/Ventriup and csGS-F/R. We identified 20 samples resistant to wheat stem rust. From the studied wheat material we identified nine samples with Lr10 gene, one with Lr34/Yr18 genes, 2 with complex genes Lr37/Sr38/Yr17 and 10 samples with Lr68 genes. The most valuable donor of sustainability is a promising line Almaly/Obriy, where 3 resistance genes Lr34/Yr18, Lr37/Sr38/Yr17, Lr68 and Yr2 were identified as well as Oktyabrina line with 2 resistance genes - Lr10 and Lr68. Our results provide an opportunity to move the selection process in Kazakhstan to a new scientific level by using molecular genetic techniques and technologies of MAS-breeding.

KEYWORDS: wheat resistant genes; rust leaf; molecular markers

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