In Vitro Hepatoprotective and Antioxidant Activities of the Leaf Ethanolic and Aqueous Extracts of Asteracantha Longifolia and Andrographis Paniculata Against Lead Acetate Induced Toxicity

Abirami¹, V. Shanmugaraju², A. Mahalakshmipriya² and K. Rajathi¹

¹Department of Biochemistry, Dr. N.G.P. Arts and Science College. KMCH Research and Educational Institutions, Kalappati Road, Coimbatroe - 48 India.

²Department of Biotechnology, Dr. N.G.P. Arts and Science College. KMCH Research and Educational Institutions, Kalappati Road, Coimbatroe - 48 India.

ABSTRACT: The present study was carried out to evaluate the in vitro hepatoprotective activity of 90% ethanolic and aqueous extracts of leafs of Asteracantha longifolia and Andrographis paniculata against lead acetate induced hepatic damage. In addition, antioxidant activity was also studied in the aqueous and ethanolic leaf extracts of Asteracantha longifolia and Andrographis paniculata. The hepatoprotective property of aqueous and ethanolic extracts of Asteracantha longifolia and Andrographis paniculata was investigated by using lead acetate to induce toxicity. The extent of lipid peroxidation (LPO), Superoxide dismutase (SOD), Catalase (CAT), Aspartate transaminase (AST), Alanine transaminase (ALT, Acid phosphatase (ACP), Alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH) were assayed in the liver homogenate. In addition the antioxidant property of aqueous and ethanolic extract was checked by assaying the activities of enzymatic antioxidants and free radical scavenging activity. The activities of liver marker enzymes Aspartate transaminase, Alanine transaminase, Acid phospatase, Alkaline phosphatase, Lactate dehydrogenase and the levels of lipid peroxidation was found to be increased during the lead acetate toxicity. Administration of aqueous and ethanolic leaf extracts of Asteracantha longifolia and Andrographis paniculata to the lead acetate induced hepatotoxicity reaches to near normal. In addition, the aqueous and ethanolic leaf extracts of Asteracantha Longifolia and Andrographis paniculata have potent antioxidant effects against several  antioxidants/ROS and capability to induce the invitro antioxidant system. The results indicate that the leafs of Asteracantha longifolia and Andrographis paniculata possess hepatoprotective property possibly because of its antioxidant activity.

KEYWORDS: Asteracantha longifolia; Andrographis paniculata; In vitro hepatoprotective; antioxidant; lead acetate toxicity

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