Secondary Metabolite Profiling of Acacia concinna

Sandip Todkar, Rohit Todkar, Vishal Kavathekar, Shantanu Kulkarni and Arvind Kulkarni    

Department of Biotechnology, Department of Microbiology, Gogate - Jogalekar College,University of Mumbai, Ratnagiri - 415 612 India.

Corresponding Author E-mail: sandip_todkar7@rediffmail.com

ABSTRACT: Secondary metabolites are a wide range of compounds from different metabolite families. Secondary metabolites are often involved in plant protection and which do not participate directly in growth and development. The aim of our study was to profile the potent secondary metabolites responsible for the antibacterial and antifungal activity. Secondary metabolites consist of varieties of chemical compounds with different structures and chemical properties. In order to get overview of secondary metabolites and to detect potent compounds responsible for the antibacterial and antifungal activity from Acacia concinna, a well suited profiling technique: Extraction with different chromatographic techniques (GC-MS, HPLC-PAD/MS) was done. The polar and non-polar methanol extracts of Acacia concinna were collected by using a suitable technique and the respective residues were subjected for HPLC-PAD/MS and GC-MS analysis. These chromatographic methods analyses ionic (charged) compounds such as Alkaloids, and non-ionic (neutral) compounds such as Flavonoids, Saponins, Tannins, Gums and Mucilage and Phenolic compounds. The profiling method reveals presence of a series of sequential compounds like 1-Methylimidazol-5-carboxaldehyde, Propanoic acid, 2- Octanamine, 3-Haptenoic acid, 3(2)-Furanone, 2-Butenedioic acid, 4-Hapten, Butanoic acid 2-propenyl ester, Pentadecanoic acid. These compounds show negative impacts on many microorganisms with its all respects. Thus it was concluded that Acacia concinna can be used as chemotherapeutic or Medicinal purpose.

KEYWORDS: Alkaloids, Flavonoids; GC-MS; Acacia concinna; Secondary Metabolites; HPLC-PAD/MS

[ HTML Full Text]