Effect of Plant Extracts on Hatching and Mortality of  Root-Knot Nematode,  Meloidogyne Incognita Larvae (in-Vitro)

Neeraj, S. R. Goel,  Anil Kumar, Gurpreet Singh and V. K. Madan

Department of Nematology, CCS Haryana Agricultural University, Hisar-125 004, India.

Corresponding Author E-mail: neeraj17051992@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2466

ABSTRACT: This research was conducted to find out the effect of aqueous extracts of different medicinal  plants against the root-knot nematode , Meloidogyne incognita in laboratory at CCSHAU , Hisar. Aqueous extracts from  plant leaves were screened for egg hatchability and nemic mortality against second stage juveniles (J₂) of M. incognita. The nematode eggs and juveniles were exposed to 24, 48 and 72 hrs to different dilutios of extracts . The dilutios as 1:5, 1:10, 1:20, 1:40 and 1:80 were prepared by diluting stock solution. As a result, M. arvesis (mentha)1:10 (v/v) dilution significantly reduced the egg hatchability and it was followed by C.longa(turmeric). At the end of 48 hrs incubation, 1:5 and 1:10 (v/v) dilutions of M. arvensis extract and C.longa produced maximum mortality in juveniles of the nematode. The results of our investigation show that five plants contain nematicidal compounds. It seems that the use of plant extracts might have increasing popularity in future as a component or ingredient of biopesticides. Therefore, further research is necessary to found out toxic compounds released by species and carried out experiments in vivo for the control of root-knot nematode , M. incognita.

KEYWORDS: C. longa(turmeric); biopesticides; Meloidogyne; juveniles;nematicidal

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