SNP of cGH gene in Egyptian chicken breeds at MspI site

Heba I. Shafey¹, Aboelhassan M. D¹, E. M. EL-Komy², Ragaa E. Abd El-karim³ and Karima. F. Mahrous¹

¹Department of Cell Biology, National Research Center, El Buhouth St., 12311 Dokki, Cairo, Egypt.

²Animal Production Department, Agriculture and Biology Research Division, National Research Centre, El Buhouth St., 12311 Dokki, Cairo, Egypt.

³Animal Production Research Institute, ARC, Ministry of Agriculture, Egypt.

Corresponding Author E-mail: l_fathy@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2414

ABSTRACT: Genetic polymorphism of cGH gene using PCR-RFLP and identification of SNP among different genotypes of Dokki-4, Inshas, El-Salam and Mandarah breeds were studied. Amplification of GH gene (intron 4) for GH2 and GH3 loci produced 563 bp fragment length. The amplified fragments (563 bp) were digested with MspI endonuclease. Loci GH2 and GH3 showed two genotype frequencies TT and TC in all breeds, while genotype CC was absent in Dokki-4 of GH2 and absent in El-Salam of GH3. Analyzed data was compared with Genbank entries of Gallus gallus (KF957977), (KF957978), (KF957979), (KF957980) and (KF957981), the sequences of detected SNP were submitted to GenBank database with the accession numbers KY176737- KY176758. Some Dokki-4 animals showed new two SNPs at positions T77C and C485T. Mandarah and Dokki-4 strains were shared in new three SNPs at positions G130A and C379T which was detected previously at KF957978. Mutation G430A of Mandarah and Dokki-4 was shown in KF957980. Eleven transitions mutations (five A/G and six C/T) and one transversion (G/C) were detected. Six transitions mutations C/T were silent mutations and position G358C was polymorphic in El-Salam.

KEYWORDS: Chicken; cGH; PCR-RFLP; SNP; MspI site

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