Genetic Biodiversity analysis of two Mitochondrial genes in Arabian and Thoroughbred Horses

Karima F. Mahrous, Heba I. Shafey, Esraa A. Balabel and Othman E. Othman

Cell Biology Department, National Research Centre, Dokki, Egypt.

DOI : http://dx.doi.org/10.13005/bbra/2413

ABSTRACT: More than 300 horse breeds or types are present all over the world and employed in different activities. This work aimed to identify genetic variations and SNPs in two effective mtDNA genes; ATP6 and ND2; among Arabian and Thoroughbred horses.The primer used in this study amplified 340-bp fragments from ATP6 gene. The results showed the presence of 6 polymorphic sites leading to the construction of 7 different haplotypes which were submitted to GenBank database with the accession numbers KX377925-KX377931. For ND2 gene, the results showed the presence of 6 polymorphic sites leading to the construction of 5 different haplotypes. The sequences of detected haplotypes of horse ND2 gene were submitted to GenBank database with the accession numbers KX396591-KX396595. Neighbor-joining trees were constructed using 24 samples for ATP gene and 20 samples for ND2 gene with 10 reference sequences and the result declared the affinities of all tested animals to Equus caballus subspecies.In conclusion, the identification of genetic variations and SNPs in horse mitochondrial genes like ATP6 and ND2 genes are of great interest because they have highly significant effect and play important roles in different characteristics associated with speed and force which constitute race performance efficiency.

KEYWORDS: Arabian horses;ATP6 gene; Genetic biodiversity; ND2 gene; genetic biodiversity Thoroughbred horses;

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