Immunohistochemical Expression of Collagen IV and MMP9 in Odontogenic Cysts and Tumors

Homeyra Mardani, Nastaran Dinarvand*, Gholamreza Jahanshahi and Elaheh Sofiyan

Department of Oral and Maxillofacial Pathology, School of Dentistry, University of Medical Sciences, Khorasgan (Isfahan) branch, Islamic Azad University,Isfahan, Iran  

DOI : http://dx.doi.org/10.13005/bbra/1911

ABSTRACT: Background the growth mechanism of odontogenic cysts and tumors along with their invasion and destructive ability can be influenced by the secretion of metalloproteinases from epithelial and mesenchymal cells. An important activity of matrix metalloproteinases is destruction of the components of basement membrane, including collagen IV. The present study was conducted to analyze the role of collagen IV and its association with matrix metalloproteinases9 in odontogenic cysts and tumors. in this study, 19 samples of keratocyst odontogenic tumor  and 11 samples of dentigerous cyst in mesenchymal stroma and odontogenic epithelium, and in basement membrane  of odontogenic epithelial neoplastic islands and basement membrane of cystic epithelium were analyzed by immunohistochemical method. collagen IV expression in keratocyst odontogenic tumors and dentigerous cysts was >50%, which showed no statistically significant difference (p=0.62). The consistency and thickness of collagen IV in dentigerous cysts were significantly higher than those of keratocyst odontogenic tumors in terms of the staining pattern of collagen IV (p=0.003, p=0.001). Also, no significant difference was observed between the epithelium of keratocyst odontogenic tumors and dentigerous cysts with regard to the expression of matrix metalloproteinase-9 (p=0.18). Expression of metalloproteinases9 in the mesenchymal stroma of keratocyst odontogenic tumors was significantly higher than dentigerous cysts (p=0.04). higher expression of matrix metalloproteinase-9 and inconsistency and low thickness of collagen IV in keratocyst odontogenic tumors may be associated with diverse biological behavior of keratocyst odontogenic tumors than dentigerous cysts.

KEYWORDS: keratocystic odontogenic tumor; dentigerous cyst; matrix metalloproteinase9; collagen IV; immunohistochemical

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