Evaluation of the Cattle Ration feed Contamination Amount With the Aspergillus Fungi by PCR-Based Technique and Based on its Gene Sequences

Sepideh Rahimi^¹ and Sahar Rahimi^²

¹Department of Plant Biotechnology,Payam Noor University (PNU), P.O.Box, 19395-3697 Tehran, Iran.

^²Department of Food Science and Technology, Pharmaceutical Sciences branch Islamic Azad University, Tehran, Iran.

Corresponding Author E-mail: rahimisahar19@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2416

ABSTRACT: Aflatoxin is carcinogenic metabolites that produced by a number of species Aspergillus section Flavi. Cattle feed contaminated with Aflatoxin endanger human and animal health. The aim of this study is to determine amount of contamination to the Aspergillus section Flavi in five main animal feed with PCR molecular technique based on ITS regions and fungal colony. In this study, 121 ration samples were used from 21 industrial animal husbandry warehouses and silage in Tehran and Alborz Province. After isolating and culture in specific sphere of yeast extract sucrose agar (YESA), isolated Aspergillus fungi were studied by macroscopic and microscopic method. For molecular identification of the Aspergillus fungi from PCR method, ITS sequencing also was used and finally grown colonies were counted to determine an amount of the fungal contamination. The results showed that 67 samples from 121 ones were positive in which represents the 55/37% contamination amount to the Aspergillus fungi, and also the highest level of fungal contamination in imported barley, wheat bran, soybean meal and corn is about 14/16%, 12/5%, 10/83% and 10/83% respectively, while the lowest fungal contamination belongs to the internal barley with 7/5%. Therefore, there is the need to develop a simple, rapid and sensitive method for the detection of Aspergillus fungi. With regard to the high fungal contamination of imported cattle feed while its amount is very low in internal products, it is better that officials try to increase internal production as well as more controlling over the production, transport and import of cattle feed.

KEYWORDS: Aspergillus fungi; Aflatoxin; PCR; ITS; Cattle feed

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