Antifungal Effect of Flavonoid Extract of Trachyspermum Ammi Plant on the Gene Expression of  Pro-Inflammatory Cytokines Such As IL-18 And TNF-Α In Articular THP-1 Monocyte/Macrophages Cells  

Maryam Sadat Abtahi^1*, Hossein Maghsoudi¹, Boshra Hatef², Parisa Marashi³, Mahnesa Vassaf³

^1*Department of Biotechnology Engineering the agricultural orientation, Payame Noor University, po box, 19395-3697, Tehran, IRAN ¹Department of Biotechnology, Payame Noor University, po box, 19395-3697, Tehran, IRAN ²Professor assistant of Neuroscience Research Center of Baqiyatallah University of Medical Science, North Artesh Highway, Second Avenue, Tehran, IRAN ³Department of Biotechnology Engineering the agricultural orientation, Payame Noor University, po box, 19395-3697, Tehran, IRAN *Corresponding author E-mail: Abtahi.ls87@yahoo.com  

ABSTRACT: The Trachyspermum ammi plant has anti-fungal properties. The aim of this study is to examine the anti-fungal  effects of this plant on the pro-inflammatory  bio-markers, which are important in the course of fungal diseases. At first the ethanol extract of the Trachyspermum ammi plant was prepared from the Iranian biological resource center. We used monocyte/macrophage cells as samples because they were similar to the cells with fungal infection. Then THP-1 monocyte/macrophages (5×10⁵ cells/well) were incubated at a humidity of 90%,  5% CO2, 37˚C for 72 h with control media alone or Trachyspermum ammi at concentrations of 10 μg/ml. One set of cells was activated for 1 h with mannose (3 mg/ml) for both reverse-transcriptase Polymerase chain reaction and real-time Polymerase chain reaction analysis of Tumor necrosis factor alpha (TNF-α), Interleukin-18 (IL-18) expression. the activated cells by mannose with  a plant extract  redused the Interleukin-18 and Tumor necrosis factor alpha expression as  activated cell by mannose with  fluconazole (p-value=0.91 for IL-18, p-valu=0.25 for TNF-α). Based on the PCR and realtime methode to analysis of gene expression,  Trachyspermum ammi plant such as the flavonoids has noticeable anti-fungal properties.

KEYWORDS: Trachyspermum ammi; THP-1 Monocyte/Macrophage; Fungal diseases

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