Low Cost Method of Activation of Micro test Plate for Image-Based Diagnostics

Shahila Parween¹, Azmi Naqvi², Dinesh C. Sharma² and Pradip Nahar³

¹Department Innovative Diagnostics, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi – 110 007, India.

²Department of Zoology, Km. Mayawati Government Girls P.G. College, Badalpur, Gautam  Buddha Nagar (U.P.) - 203207.

³New Idea Lab, C-202, Ramkrishnan Cooperative Group Housing Society, Plot-12, Sector 23, Dwarka, New Delhi 110077, India.

 Corresponding Author E-mail:  azminqv@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2504

ABSTRACT: In this communication, we report the activation of micro test plates (polypropylene plate) by introducing an active functional group through photolinker either by artificial UV irradiation or by sunlight exposure for image based diagnostics. This simple and unique technique leads to specificity and sensitivity of the assay.  The, sunlight mediated activation of micro test plate can be a  potential alternative to artificial UV light, as the required sunlight intensity for activation  is available in most parts of the world and also this method is cheap, eco friendly, and is a clean energy source. Optimum activation was obtained at 90,000 lux, beyond this sunlight exposure produced almost uniform activation. Thus, this rapid and simple method of surface activation could be used for the immobilization of different biomolecules such as protein, DNA, or carbohydrate which can be used for different purposes including ELISA and other related techniques on micro test plate.

KEYWORDS: Photolinker; Immobilization; Microtest plate; Immobilization

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