Growth Characteristics of Indian and Nigerian Chlorella Pyrenoidosa used as food Supplement

Onwurah Christian, Shahana Majumder and Pankaj Taneja  

Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida.

Corresponding Author E-mail: pankajtnj@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2516

ABSTRACT: Chlorella pyrenoidosa from National Collection of Industrial Microorganism (NCIM) Pune, India and that from Department of Biotechnology, University of Nigeria Nsukka (UNN) were cultivated using Fog’s media. The two samples in Erlenmeyer conical flasks were incubated at temperature of 28⁰C at 12hrs. Photo and 12hrs dark periods at 2500 Lux using light from fluorescent tubes. The cultivation was carried out for the period of 30 days. The growth rates were assessed using spectrophotometer at wave length of 660nm at 7days interval. The result of the assessment showed that Chlorella pyrenoidosa from NCIM Pune, India had initial optical density (O.D.) of 0.1024 while that from UNN Nigeria was 0.0956, while the final India sample was 1.0462 and that from Nigeria was 0.788 respectively. The result of cell count using Neubauer hemocytometer showed that Cfu for C. pyrenoidosa from India was 1.8 x 10⁶ while that from Nigeria was 1.0 x 10⁶ per ml respectively. Total Chlorophyll was equally determined using 80% acetone as solvent that from Pune, India had a mean of total Chlorophyll of 12.42µg/ml while that from Nigeria 12.22µg/ml respectively. All the result obtained were subjected statistical using t-test and they showed significant different between India specie and Nigeria, since t cal< t tab at (P< 0.05). The growth rate measured with spectrophotometer showed that the Indian sample had higher O.D. than Nigerian which may be due to Temporal, environmental genetic acclimatization changes and local geological condition can also be the reason for variations between the same species from different region Temperate India and Tropical Nigeria.

KEYWORDS: Chlorella pyrenoidosa; Fog’s media; Food Supplement optical density; Spectrophotometer;

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