RsbA Gene's Confirmation in P. Mirabilis With PCR Method in Patients With Urinary Tract Infection in Province of Qorveh

Samira Dehnavi and Bahareh Rahimian Zarif  

Department of  Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.

Corresponding Author E-mail: Bahareh_r_z@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2511

ABSTRACT: Urinary tract infections are among prevalent diseases which can happen in women and men in different age groups. Proteus mirabilis is considered as the cause of 10 percent of urinary tract infections and is the fifth common nosocomial urinary tract infection. A major problem in nosocomial infections is emergence of multidrug resistant species. It is assumed that rsbA in Proteus mirabilis is an engaged gene in this process. Consequently, in this study we have assessed the relation between the presence of this gene and antibiotic resistance. 40 urine sample which were diagnosed to be positive for Proteus mirabilis using differential test, were assessed for antibiotic resistance to beta lactam antibiotics using diffusion disk and were analyzed using PCR for rsbA gene. Results of antibiotic resistance and presence of rsbA gene were analyzed using Pearson correlation statistical test. Results showed 60%, 50%, 34%, 44%, and 20% antibiotic resistance for penicillin, cefotaxime, carbapenem, cefipenem, and ceftriaxone respectively. RsbA gene was present in 67% of samples. Pearson statistical analysis showed a statistically significant relationship between antibiotic resistance and rsbA gene.

KEYWORDS: antibiotic resistance; Proteus mirabilis; rsbA gene; urinary infection

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