Production of Xylanase from A Mixed Culture System of Acetobacter Xylinum and Cellulomonas Uda in Submerged Fermentation

Preethi Murugan, Preethi Jampala, Swathy Ramanujam and Kiran Babu Uppuluri*

Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur-613401, Tamil Nadu, India

ABSTRACT: Xylanase (endo-1, 4-β-xylanase) is an industrially important enzyme that hydrolyses hemicellulose, xylan into xyloligosaccharides, xylotriose, xylobiose and xylose. Commonly xylanase can be produced by a wide variety of microorganisms. It is widely used for hemicellulosic hydrolysis especially in biofuel production, textile and paper manufacture. The present work aims the production of xylanase by co-culturing Acetobacter xylinum NCIM 2526 and Cellulomonas uda NCIM 2353 utilizing a novel and cheap lignocellulosic substrate, Prosopis juliflora under submerged fermentation (SmF). This traditional approach using a mixed microbial culture and cheap substrates can reduce the enzyme production cost. Growth patterns and xylanase production profiles of chosen bacteria were studied in both mono and mixed cultures.   Mixed culture of Acetobacter xylinum and Cellulomonas uda was produced higher xylanase titers than the mono-cultures. Maximum xylanase activity, 5.85 U/ml was obtained using 3% pods in 72 hours of fermentation with the mixed microbial culture. This may be due to efficient use of substrates as compared to their respective mono-cultures.

KEYWORDS: Acetobacter xylinum; Cellulomonas uda; Prosopis juliflora; Xylanase; Mixed culture

[ HTML Full Text]