Volume 14, number 2
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Genomic DNA Extraction for Molecular Identification of Endophytic Fungi: an Easy and Efficient Protocol

Farzana Gul, Anwar Hussain, Gul Jan and Muhammad Hamayun

Department of Botany, Abdul Wali Khan University Mardan, Pakistan.

Corresponding Author E-mail: hamayun@awkum.edu.pk

DOI : http://dx.doi.org/10.13005/bbra/2492

ABSTRACT: Presence of tough chitinous cell wall in fungi is a challenge for cell lysis and subsequent DNA isolation which has several downstream applications. We have developed an efficient protocol for extracting genomic DNA from endophytic fungi, which was isolated from different parts of Solanum xanthocarpum L. The procedure used was based on sodium dodecyl sulfate/ phenol/chloroform extraction method to remove polysaccharides and protein. The isolated genomic DNA was used to successfully amplify ITS region. The amplified ITS region was sequenced and homology of its sequence was used for confirming identity of the fungal endophyte. This rapid and inexpensive method can efficiently isolate high quality and quantity of genomic DNA suitable for PCR amplification and sequencing.

KEYWORDS: Genomic DNA extraction; endophytic fungi

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Gul F, Hussain A, Jan G, Hamayun M. Genomic DNA Extraction for Molecular Identification of Endophytic Fungi: an Easy and Efficient Protocol. Biosci Biotech Res Asia 2017;14(2).
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Gul F, Hussain A, Jan G, Hamayun M. Genomic DNA Extraction for Molecular Identification of Endophytic Fungi: an Easy and Efficient Protocol. Biosci Biotech Res Asia 2017;14(2). Available from: https://www.biotech-asia.org/?p=25829

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