In vitro Regeneration of Alstroemeria cv. ‘Balance’ Based on Direct Organogenesis

Maryam Beigi Harchegani^1, Hossein Nazarian², Mahmoud Otroshy³, Mohammad Ali Ebrahimi⁴ and Ali Motamedi⁵

1,2Department of Agricultural Biotechnology, Payame Noor University, Karaj, I. R. Iran.

3Department of Tissue Culture, Agricultural Biotechnology Research Institute of Iran, Najaf Abad, Isfahan, Iran.

4Department of Agricultural Biotechnology, Payame Noor University, Tehran, I. R. Iran.

5Faculty of Agriculture, Shahid Beheshti University, Tehran, Iran.

Corresponding Author Email: alimotamedi1987@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2479

ABSTRACT: The present study aimed at improving the efficiency of the in vitro regeneration of Alstroemeria cv. ‘Balance’ protocol through direct organogenesis technique using three different origins of explants (nodal stem, rhizome apical bud, rhizome segments) as two separate factorial experiments with completely randomized design with six replications which were implemented in three stages. Firstly, Direct regeneration, including two factorial experiments to induce regeneration in organogenesis media by utilizing the four NAA concentrations (0, 0.5, 1, 2 mg/l) combined with five BAP concentrations (0, 0.5, 1, 1.5, 2 mg/l) in the first experiment, and TDZ concentrations (0, 0.5, 10 mg/l) in combination with three IBA concentrations (0, 1, 2 mg/l) in the second experiment. Secondly, shoot regeneration and elongation of stems in regenerated buds in the MS basal medium and finally, rooting of the regenerated shoots in the root induction mediums by NAA and IBA. Results of regeneration experiment revealed that, in the culture medium containing 10 mg/l TDZ in combination with 2 mg/l IBA and rhizome apical bud explants, the maximum rates of regeneration percentage, the highest number and length of the shoots were produced. Resulted shoots produced the greatest number of roots and rhizomes in the culture medium comprising 1 mg/l NAA in combination with 2 mg/l IBA as auxins. From the two tested explants, rhizome apical bud, was known as the best explants for shoot regeneration obtained plantlets successfully adapted to environmental conditions and were transferred to the greenhouse. Results of current study indicated that Alstroemeria can be produced through direct organogenesis.

KEYWORDS: Alstroemeria; direct organogenesis; tissue culture; plant growth regulator; explants

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