In vitro Regeneration of Alstroemeria cv. ‘Balance’ by Indirect Organogenesis

Hossein Nazarian¹, Maryam Beigi Harchegani¹, Mahmoud Otroshy² and Ali Motamedi³

¹Department of Agricultural Biotechnology, Payame Noor University, Karaj, I. R. Iran.

²Department of Tissue Culture, Agricultural Biotechnology Research Institute, Najaf Abad, Isfahan, Iran.

³Faculty of Agriculture, Shahid Beheshti University, Tehran, Iran.

Corresponding Author Email: alimotamedi1987@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2484

ABSTRACT: This study was designed in order to optimize the indirect organogenesis (during callus induction and regeneration) of Alstroemeria cv. ‘Balance’ through tissue culture technique in two phases; the first stage: callus induction by rhizome segments, leaf and nodal stem which in the start, callus formation media were examined using two types of auxins; 2,4-D and NAA and a cytokinin; BAP in four different experimentations. In the second stage, calli derived from rhizome segments and nodal stem explants were transferred to regeneration media. The results revealed that 2,4-D in combination with BAP in the rhizome segments and nodal stem explants were efficient as compared to NAA. The highest yield of callus formation was also obtained in the rhizome segments explants. According to the results, it can be suggested that NAA as auxin, does not have direct positive effect on cell division in Alstroemeria. The 2,4-D is toxic at high concentrations and may bring about cell death. Eventually, the composition of 0.5 mg/l NAA with 3 mg/l BAP and callus derived from nodal stem explants may be introduced as the best combination for regeneration. These results indicate the necessity of the BAP cytokinin presence for regeneration. In addition, the maximum length of the shoot was obtained from combination of BAP with nodal stem explants, without the presence of NAA.

KEYWORDS: Alstroemeria; callus induction; organogenesis; PGRs;  regeneration

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