Identification of QTL Associated With Grain Length, Grain Width and L/B Ratio in Double Haploid and Recombinant Inbred Line Population in Rice (Oryza Sativa L.)

Krityanand Kumar Mahatman^1,3, Rupesh Deshmukh¹, Anshuman Tiwari¹, Mahesh Chandra Yadav², Jyoti Kumari³ and Satish B. Verulkar¹

¹Department of Plant Molecular Biology and Biotechnology,  College of Agriculture, Indira Gandhi Agricultural University, Raipur-492006, CG, India.

²Division of Plant Genomic Resources, National Bureau of Plant Genetic Resources, Indian Council of Agricultural Research (ICAR), PUSA, New Delhi-110012, India.

³Division of Germplasm Evaluation, National Bureau of Plant Genetic Resources, Indian Council of Agricultural Research (ICAR), PUSA, New Delhi-110012, India.

Corresponding Author E-mail: krityabt@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2519

ABSTRACT: With the substantial increase in food grain production, much of the emphasis has been put for enhanced grain quality traits. Most of the grain qualities traits are polygenic in nature. Identification of QTLs and markers linked to these QTLs can substantially enhance the selection efficiency. In this study, an effort has been made to identify QTLs for these traits. For this purpose two mapping population, RIL Safri17 x Kranti and DH population CT-993-5-10-1-M and IR 62266-42-6-2 were used and evaluated under field conditions. DNA from 112 lines of RIL was extracted, quantified and diluted to the appropriate concentration for PCR-based amplification. Fifty markers were used for detecting parental polymorphism. Five markers exhibiting polymorphism were further used for developing marker profile on the complete set of RIL. This data along with genotyping data for this population was used for QTLs analysis using single marker ‘t’ test. RM 110, RM 202 and RM 212 were associated with grain length, RM 84 and RM 539 was associated with grain width, and RM 163, RM 202, RM 247 and RM 80 were associated to be L/B ratio. For DH population MAPMAKER/ QTL 1.1 was used for interval mapping and to estimate the percentage of total phenotypic variation. A threshold of LOD> 2.4 was used per test to claim the presence of QTL. A total of 8 QTLs were detected for grain width and grain L/B ratio. For grain width 3 QTLs detected on chromosome number 2, 3, 6 which shows 11 percent phenotyping variation. For L/B ratio 5 QTLs were detected, which were present on chromosome 2 3, 6 having 9 to 12.5 percent phenotypic variation. For grain length no QTLS detected.

KEYWORDS: Grain Length; Grain Width; Grain L/B ratio;Rice;  QTLs

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