Study of Water Quality and Biochemical Characterization of Bacterial Isolates from Water Samples of Ponnagi Area in Krishna District

Jyothi Kshatri¹, C. V. Rao¹ and Vijaya Saradhi Settaluri²

¹Chukkapalli Vari Street, Labbipet,Vijayawada-520010, Andhra Pradesh, India.

²Department of Biotechnology, K L University, Vaddeswaram-522502, Andhra Pradesh, India.

Corresponding Author E-mail: snehabiotech@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2551

ABSTRACT: Aquaculture also known as fish farming is one of the methods for breeding rearing and harvesting of freshwater and marine species of fish and shellfish, in ponds, rivers, lakes and oceans.  In this article an attempt has been put forth to study and apply techniques that could help in improving the water quality in aquaculture ponds and to further understand the role played by enzymes and probiotics by means of bio remedial procedures and protocols. Aquaculture productivity needs to be improved to cater the ever-increasing demand, no doubt, but simultaneously a proactive role for environment protection is required. The gathering of organic wastes worsens the water quality and reduces the level of dissolved oxygen in the fish aquaculture ponds. This further increases the formation of toxic metabolites such as ammonia (NH₃), nitrite (NO₂^-) and hydrogen sulphide (H₂S). Hence, NH₄⁺ or NO₃^- removal processes (nitrification and denitrification) become essential for the pond water quality. This can be carried out by applying different types of nitrifying and denitrifying bacteria such as Nitrosomonas, Nitrobacter and Alcaligenes. In the present investigation, 2 earthen ponds were selected from Ponnagi near Eluru in Krishna district, Andhra Pradesh, India. One pond (Pond A) was treated with probiotics having Nitrosomonas, Nitrobacter and Alcaligenes sps. And the other  pond (Pond B) was kept as control. During the culture period, the water samples were collected from probiotics treated and control ponds for analysis of water quality parameters. The current study is aimed to focus on the changes in water quality and biochemical characterization of bacterial isolates from water samples of Ponnagi area in probiotic treated ponds and to compare the results with ponds not treated with probiotics.

KEYWORDS: Aquaculture characterization;Microorganisms; ponds; Probiotics; quality;

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