Application of Green Synthesized Iron Nanoparticles for Enhanced Antimicrobial Activity of Selected Traditional and Commonly Exploited Drug Amoxicillin Against Streptococcus mutans

Gudaniya Kasthuri, A. Neethi Reddy, P. Manasa Roopa and Deveeka. k. Zamare

Sreenidhi Institute of Science and Technology Department of Biotechnology.

DOI : http://dx.doi.org/10.13005/bbra/2552

ABSTRACT: The development of drug resistance in pathogens due to over exploitation of the drugs is urging the need of finding alternative method for controlling the growth of these pathogens. The dental biofilms development leads to the dental caries which when neglected can be serious. The oral biofilm is a complex colonization of different pathogens, whereas Streptococcus mutans paves the path for the formation of these stubborn biofilms by makeing use of dietary sugars and accumulates on tooth surface through exopolysaccharides (EPS). The current life style and eating habits are favoring the plaque development leading in to dental caries. Here we amide to eliminate the dread of development of drug resistance in to the pathogens by mechanical damage and combination of treatment as an alternate option for destruction of Streptococcus mutans. This was accomplished by the application of Iron Nanoparticles (FeNP). It was observed that the antimicrobial activity of natural and synthetic drugs can be enhanced by the synergy of FeNP. The popular natural products like Syzygium aromaticum (Clove) buds, Azadirachta indica (Neem) leaves and Camellia sinensis (Green tea) leaves were tested for their activity against the Streptococcus mutans. The enhanced antimicrobial activity were tested by treating  Streptococcus mutans with different combination of treatments like only plant extract, Plant extract with FeNP, plant extract with FeNP and Amoxicillin to obtain a comparative analysis of its effects. The study showed that the activity of antimicrobial agent can be enhanced when added with FeNP. The FeNP were green synthesized from the extracts of Syzygium aromaticum (Clove) buds, Azadirachta indica (Neem) leaves and Camellia sinensis (Green tea) leaves and was characterized by UV,FTIR and SEM studiesand zeta potential studies.

KEYWORDS: Application of iron nanoparticles;antimicrobial activity;  Dental biofilm

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