Volume 14, number 3
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Expansion of Cord Blood Hematopoietic Stem Cells on the Amniotic Membrane Derived Mesenchymal Stem Cells

Nooshin Barikrow1, Naser Amirizadeh2, Nasim Hayati Roodbari1 and Mahin Nikougoftar2  

1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2Blood Transfusion Research center, High institute for education and research in Transfusion Medicine, Tehran, Iran.

Corresponding Author E-mail: n.amirizadeh@ibto.ir

DOI : http://dx.doi.org/10.13005/bbra/2526

ABSTRACT: Because of insufficient number of umbilical cord blood hematopoietic stem cells (UCB-HSCs), expansion of these cells seems to be important for clinical application in adults. The aim of this study was to co-culture of UCB-HSCs with the amniotic membrane derived mesenchymal stem cells (AMMSCs) as a feeder layer in order to expand hematopoietic stem cells (HSCs). UCBs and amniotic membrane were collected from concern mothers. Ex vivo culture of UCB-HSCs were performed in four culture conditions: cytokine cocktail with MSCs feeder layer, cytokine cocktail, stem cell factor, and co-culture with MSCs without any cytokine. The number of total nucleated cells (TNC) was counted by hemocytometer. The HSC count and immunophenotyping of Mesenchymal stem cells (MSCs) and expanded HSC were evaluated by flow cytometry. Colony forming unit (CFU) assay was used to evaluate the potential of expanded HSCs for production of different lineage colonies. The mean fold changes of total nucleated cells (TNC) and  CD34+ cells in the cytokine culture with feeder layer were higher than the cytokine culture without MSCs. However, in the co-culture system without cytokine, TNC and CD34+ cell numbers were increased up to 8 folds, but cell viability was more than 80% and differentiation rate was low. Our results demonstrated that we could increase the number of CD34+ cells of UCB that were used as primary HSC for transplantation.

KEYWORDS: Amnion; Cord Blood Stem Cell Hematopoietic Stem Cells; Mesenchymal Stromal Cells;Tissue Expansion; Transplantation

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Barikrow N, Amirizadeh N, Roodbari N. H. Nikougoftar M. Expansion of Cord Blood Hematopoietic Stem Cells on the Amniotic Membrane Derived Mesenchymal Stem Cells. Biosci Biotech Res Asia 2017;14(3).
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Barikrow N, Amirizadeh N, Roodbari N. H. Nikougoftar M. Expansion of Cord Blood Hematopoietic Stem Cells on the Amniotic Membrane Derived Mesenchymal Stem Cells. Biosci Biotech Res Asia 2017;14(3). Available from: https://www.biotech-asia.org/?p=27664

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