Diversity of Tropical Marine Macroalgae from Coastal Area of Sayang Heulang, West Java Indonesia

Dimas Frananta Simatupang¹, Jannah Sofi Yanty^1,3, Yanti Rachmayanti¹, Fida Madayanti Warganegara¹ and Akhmaloka^1,2

¹Biochemistry Research Group, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Indonesia.

²Department of Chemistry, Faculty of Science and Computer, Universitas Pertamina, Indonesia.

³Department of Medical Laboratory Techmologgy, Politeknik Kesehatan Bali, Indonesia.

Corresponding Author E-mail: loka@chem.itb.ac.id

DOI : http://dx.doi.org/10.13005/bbra/2616

ABSTRACT: Genetic diversity of nine marine macroalgae from coastal area of Sayang Heulang were assessed based  on phenotypic and ribotyping analysis. Morphology of the macroalgaes were classified based on the color of the organism such as red (4 samples), brown (3 samples) and green (2 samples). Genomic DNA from the organisms were isolated using CTAB method with slight modification. The 18S rRNA gene amplified by PCR method using standar primers for V4 region of 18S rRNA gene. The amplicons were sequenced and analysed. The results showed that all the sequences are closed to V4 region of 18S rRNA gene. Comparison among the nine sequences showed a variation of homology. The best homology was shown by the sequence of SB and TC (99,1%), both are brown color macroalgea. The lowest homology was shown by GU (red color) and TC (brown color) with the homology at around 64,6%. Further analysis by comparing among the same color and the cross color showed that the same color does not show genetically close each other. Phylogenic analysis showed that the three brown colors are closed each other, however the rest are seen different and far away each other.

KEYWORDS: Ribotyping Analysis; Tropical Macroalgae; V4 Region of 18S  rRNA Gene

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