DPP-IV Inhibitory Potential of Methanolic Extract of Pueraria Tuberosa in Liver of Alloxan Induced Diabetic Model

Shivani Srivastava, Durgavati Yadav and Yamini Bhusan Tripathi

Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, U.P, India.

Corresponding Author E-mail: yaminibiochemist6@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2602

ABSTRACT: DPP-IV is usually found to be over expressed in many pathological conditions of Liver via disturbing immune system, lipid accumulation, ECM degradation etc. The main objective of this work was to explore the effect of methanolic extract of Pueraria tuberosa (PTME) against Alloxan induced liver damage with respect to its potential of inhibiting DPP-IV activity. PTME was prepared through continuous soxhlet extractor. Alloxan injections were given to the same age group with weight range of 80-100 g Charles foster albino male rats at the dosage of 120mg/kg bw.  Rats were divided into five groups. Group 1 was given with PTME dose of  20mg/100 g bw for 7 days, Group 2 for 14 days, Group 3 for 30 days, Group 4 for 40 days and Group 5 taken as an Alloxan control.  Animals were sacrificed at their respective time along with the normal rats. DPP-IV activity in liver homogenates were done through the kit based on fluorescence ELISA. After treatment with PTME (20 mg/100 g bw) at different time intervals, the alloxan induced stress enhanced DPP-IV activity in liver significantly decreased in a time dependent mannerin. This short study provides an idea, how PTME protects liver via its potential role as DPP-IV inhibitor. But it needs further deep study to reveal the overall mechanism at pathological, clinical and molecular basis in different models of liver diseases.

KEYWORDS: Alloxan; DPP-IV; Liver; PTME

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