Cultural, Anastomosis, and Universally Primed PCR Typing of Rhizoctonia Solani from Potato

Mousa A. Alghuthaymi

Department of Biology, Science and Humanities College, Alquwayiyah, Shaqra University, Saudi Arabia.

Corresponding Author E-mail: malghuthaymi@su.edu.sa

DOI : http://dx.doi.org/10.13005/bbra/2614

ABSTRACT: Forty two isolates of Rhizoctonia solani were recovered from potato in four agro-ecological regions of potato in Saudi Arabia were studied by using anastomosis typing, morphological characters, and molecular method. Almost of isolates sampled from potato cultures belonged to AG-1, AG-3, and AG-7.  RP1, RP2, RP3, and RP4 are bridging isolates, they might be classified into another anastomosis groups (AGs) or perhaps might be new anastomosis groups. Universally primed PCR (UP-PCR) fingerprinting was used to evaluate genetic diversity of R. solani AGs infecting potato and other hosts. The majority of the isolates representing various AGs were grouped together into different sub-clusters using all three primers. Molecular groups of the isolates did not correspond to agro-ecological regions or states and crops of the origin. In the current study, the UP-PCR markers could not identify and clearly differentiate the isolates of R. solani. There was no relationship between the geographic origin of the isolates and clustering of isolates based on the genetic diversity. Based on cultural characterization, anastomosis group typing and UP-PCR, no clear-cut conclusions about non-identified anastomosis groups from potato.

KEYWORDS: Anastomosis Typing; Potato; Thanatephorus Cucumeris; Sclerotia; UP-PCR

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