Investigation on Growth of Oil Degrading Thermophilic Bacteria Isolated from Hot Spring

Iqbal Ansari¹, Ritesh Kumar², Muniyan Sundararajan², Deblina Maiti¹, Mohd Ashraf Rather³ and Meemshad Ali⁴

¹Academy of Scientific and Innovative Research (AcSIR), CSIR-CIMFR, Dhanbad, Jharkhand, India.

²Scientist, CSIR-CIMFR, Dhanbad-826015, India.

³Department of Fisheries Biology, College of Fisheries, Ratnagiri, India.

⁴Department of Biotechnology, VBU, Hazaribagh, India.

Corresponding Author E-mail id:iqbal.cimfr@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2689

ABSTRACT: Oil spills are one of the key concerns of oil industry. The main distress of environmental specialists is the hazard to the marine ecosystem, caused due to offshore oil spills. In the present study the oil degrading potential of isolated bacteria on different media composition was carried out. From the study, it has been found that oil degrading thermophilic bacteria are capable of degrading soyabean oil, olive oil, tween 20, glycerol and crude petroleum oil. The culture of thermophilic was performed in thermus agar media and it was observed from the experimental study that the growth of thermophilic bacteria was moderately good at the range of 50^oC to 60^oC and 60^oC to 70^oC but declines after 70^oC; no growth was observed in the range of 25^oC to 50^oC. It has also detected that the decaying capability of the thermophilic bacteria in Olive oil is initially better than soyabean oil during first six hours culture afterward its performance is better in soyabean oil than Olive oil. The present study is of special environmental significance as it can be efficiently used for bioremediation of oil polluted water.

KEYWORDS: Broth Media; Crude Petroleum Oil; Hot Water Springs; Thermophilic Bacteria

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