Effect of wounding and stress on proteinase inhibitor profile of Zizyphus jujuba leaves

J. Varghese and M. B. Patil*

Post Graduate Teaching Department of Biochemistry, L.I.T. Premises, Nagpur University, Nagpur - 440 033 (India)

ABSTRACT: Effect of wounding and stress during the growth phase of Zizyphus jujuba showed quantitative changes in proteinase inhibitor (PI) pattern in the leaves. The PI level was found to increase when 30 days old plants were artificially wounded. The PI was found to be maximum at 16 hr after wounding, which was simultaneously followed by an increase in total protein content in leaves of Z. jujuba. Similarly, the PI level in the leaves was also found to increase when the plants were subjected to high osmotic (NaCl and mannitol) and drought stress treatment. It was also observed that the progressive increase in the osmotic stress led to the accumulation of proline in the leaves.

KEYWORDS: Zizyphus; proteinase inhibitor; wounding; drought stress; osmotic stress

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