Study of Flavonoid and Antioxidant Activity of Saponaria Officinalis L. that Occurs in Buryatia

Galina Batoevna Еndonova, Tatiana Petrovna Antsupova, Sesegma Dashievna Zhamsаranova, Dandar Vladimirovich Lygdenov

East Siberia State University of Technology and Management, 40V Klyuchevskaya ul., Ulan-Ude, Russia, 670013

DOI : http://dx.doi.org/10.13005/bbra/1869

ABSTRACT: The aim was to study the flavonoid composition of Saponaria officinalis, occurring on the territory of Buryatia, and evaluation of the antioxidant activity of the extracts of the aerial organs. The highest content of total flavonoids was noted in the leaves and inflorescences of Saponaria officinalis, gathered in sparse pine forest (near the city of Ulan-Ude). By the high performance liquid chromatography (HPLC) method we conducted a qualitative and quantitative analysis of the aerial part of Saponaria officinalis (S.officinalis). In the alcoholic extraction we identified 5 compounds of aglycone and glycosidic nature; apigenin derivatives are dominant (43.95 mg / g). The highest antioxidant activity of the extracts was in the leaves and inflorescence of Saponaria officinalis collected on abandoned sites near the village. Selenginsk (Kabansky region). To maximize the extraction of antioxidants from the plant material we studied various extractants and different variants of relations plant extract : buffer solution. We chose an optimum ratio of the plant extract and buffer solution 1: 9. With increasing the concentration of the plant extract at a dilution with buffer solurion a total content of water-soluble antioxidants increased and fat-soluble - decreased.

KEYWORDS: Saponaria officinalis; flavonoids; antioxidant activity; lipids

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