In Vitro Analysis Of Anti-Cancer Activity Of Oroxylum Indicum With The Combination Of Catharanthus Roseus, Commiphora Mukul, And Cynodon Dactylon In Dla Transplanted Swiss Albino Mice

Smitha Sam and N. Ganesh

Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal - 462 001 (India)

ABSTRACT: A comparative study of anticancer efficacy of Oroxylum indicum with the combination of Catharanthus roseus, Commiphora mukul and Cynodon dactylon in DLA (Daltons Lymphoma Ascities tumor cell lines) transplanted Swiss albino (Mus muculus) mice by in vitro methods were done. Swiss albino male mice (32 in number) treated with DLA cell lines were used in the present investigation. Along with this, sweet potatoes, which were transformed with Agrobacterium tumifascience and treated with different concentrations of medicinal plants like Oroxylum indicum, Catharanthus roseus, Commiphora mukul and Cynodon dactylon and were developed with tumors, after 21 days was also used for the study. The total number of tumors per disc was counted and DNA was isolated from both the transformed sweet potatoes and tumor cells of the cancerous mice. They were loaded in agarose gel and checked the percentage of genotoxicity by ‘DNA laddering method’ by comparing the molecular weight with EcoR1 Hind III Double Digested marker. While studying the results of each experiment, it was found that Oroxylum indicum, the unexplored ayurvedic medicinal plant, is a real promise in the cancer treatment in near future with low cost and high mode of activity.

KEYWORDS: Oroxylum indicum; Catharanthus roseus; Commiphora mukul; Cynodon dactylon

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