A Strategic Production Improvement of Streptomyces Beta Glucanase Enzymes with Aid of Codon Optimization and Heterologous Expression

Lekshmi K. Edison¹, Vipin Mohan Dan¹, Reji S. R¹ and Pradeep N. S²*

¹Microbiology Division, KSCSTE-Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala- 695 562, India

²KSCSTE-Malabar Botanical Garden and Institute of Plant Sciences, Kozhikode, Kerala- 673014, India

Corresponding Author E-mail : drnspradeep@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2862

ABSTRACT: β-glucan rich cereals such as barley and oats serve as a raw material in breweries and also used as an animal feed. Digestion of β-glucan is often a major hurdle, thus providing exogenous enzyme β-glucanase serves as an option. The present study takes an effort for the expression and over production of β-glucanase genes from Streptomyces sp in E. coli. The exo-β-1,4-glucanase and endo-β-1,3-glucanase encoding genes were isolated and codon optimized and significant-high expression levels were obtained in E. coli strain. The expressed enzymes showed broad pH stability, good thermostability, and better affinity towards the barley β-glucan substrate. The study implies that heterologous expression with codon optimization strategy enhances the production of Streptomyces origin beta-glucanase enzymes with prominent physio-chemical properties for efficient beta-glucan degradation.

KEYWORDS: Animal Feeds; Brewing; Beta Glucanase; Industrial Enzymes; Production Enhancement; Streptomyces; Western Ghats

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