A Strategic Production Improvement of Streptomyces Beta Glucanase Enzymes with Aid of Codon Optimization and Heterologous Expression
Lekshmi K. Edison1, Vipin Mohan Dan1, Reji S. R1 and Pradeep N. S2*
1Microbiology Division, KSCSTE-Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala- 695 562, India
2KSCSTE-Malabar Botanical Garden and Institute of Plant Sciences, Kozhikode, Kerala- 673014, India
Corresponding Author E-mail : drnspradeep@gmail.com
DOI : http://dx.doi.org/10.13005/bbra/2862
ABSTRACT: β-glucan rich cereals such as barley and oats serve as a raw material in breweries and also used as an animal feed. Digestion of β-glucan is often a major hurdle, thus providing exogenous enzyme β-glucanase serves as an option. The present study takes an effort for the expression and over production of β-glucanase genes from Streptomyces sp in E. coli. The exo-β-1,4-glucanase and endo-β-1,3-glucanase encoding genes were isolated and codon optimized and significant-high expression levels were obtained in E. coli strain. The expressed enzymes showed broad pH stability, good thermostability, and better affinity towards the barley β-glucan substrate. The study implies that heterologous expression with codon optimization strategy enhances the production of Streptomyces origin beta-glucanase enzymes with prominent physio-chemical properties for efficient beta-glucan degradation.
KEYWORDS: Animal Feeds; Brewing; Beta Glucanase; Industrial Enzymes; Production Enhancement; Streptomyces; Western Ghats
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Copy the following to cite this article: Edison L. K, Dan V. M, Reji S. R, Pradeep N. S. A Strategic Production Improvement of Streptomyces Beta glucanase Enzymes with Aid of Codon Optimization and Heterologous Expression. Biosci Biotech Res Asia 2020;17(3). |
Copy the following to cite this URL: Edison L. K, Dan V. M, Reji S. R, Pradeep N. S. A Strategic Production Improvement of Streptomyces Beta glucanase Enzymes with Aid of Codon Optimization and Heterologous Expression. Biosci Biotech Res Asia 2020;17(3). Available from: 0 |