Volume 17, number 3
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Genotype-Dependent in Vitro Regeneration Assessment from Decapitated Embryonal Axis and Stem-Node Explants among Selected Pigeonpea Varieties

Vandana Kashyap1, Bijaya Ketan Sarangi2, Manoj Kumar Yadav3 and Dinesh Yadav1*

1Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh- 273 009, India

2Environmental Biotechnology and Genomics Division, National Environmental Engineering research Institute- CSIR, Nehru Marg, Nagpur-440 020, India

3College of Agriculture, Department of Biotechnology, S.V.P university of Agriculture and Technology, Meerut 250 110, India

Corresponding Author E-mail : dinesh_yad@rediffmail.com

DOI : http://dx.doi.org/10.13005/bbra/2847

ABSTRACT: The inherent regeneration ability among selected varieties of pigeonpea using decapitated embryonal axis and stem-node explants in the presence of different growth regulators were assessed for multiple shoot bud induction. Among three different hormones namely BAP, Kinetin and TDZ tested for in vitro regeneration at different concentration for decapitated embryonal axis explants, BAP was found to be comparatively better as evident from number of buds per explants.  IPA-242 variety was found to be the best for direct organogenesis resulting in the formation of 10 buds when subjected to MS Medium supplemented with 2.0 mgL-1 of BAP. Under treatment with different concentration of TDZ, Pusa-9 revealed 10 shoot buds with 0.15 mgL-1 of TDZ. The overall response of these varieties at different concentration of kinetin was very poor. With stem-node explants under variable concentration of BAP, IPA-3088 performed best showing 17 buds per explants. Similarly Pusa-9 and IPA 3088 revealed maximum regeneration ability forming 10 and 8 buds under TDZ and kinetin respectively. NAA was found to be effective growth regulator for rooting of shoots regenerated both from decapitated embryonal axis and stem-node explants.

KEYWORDS: Decapitated Embryonal Axis, Explant; Multiple Shoot Bud Induction; Pigeonpea (Cajanus cajan L. Millsp.); Regeneration; Stem-Node

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Kashyap V, Sarangi B. K, Yadav M. K, Yadav D. Genotype-Dependent in Vitro Regeneration Assessment from Decapitated Embryonal Axis and Stem-Node Explants among Selected Pigeonpea Varieties. Biosci Biotech Res Asia 2020;17(3).
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Kashyap V, Sarangi B. K, Yadav M. K, Yadav D. Genotype-Dependent in Vitro Regeneration Assessment from Decapitated Embryonal Axis and Stem-Node Explants among Selected Pigeonpea Varieties. Biosci Biotech Res Asia 2020;17(3). Available from: https://bit.ly/3giTwHN

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