Molecular Fingerprinting of Indian Medicinal Tree Sara Asoca using RAPD Markers

Shailendra Singh Yadav and Ashwini A. Waoo

Department of Biotechnology, Faculty of Life Sciences and Technology, AKS University, SATNA, MP, India

Corresponding Author E-mail: biotech.yadav0@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2874

ABSTRACT:

Saraca asoca is an important medicinal tree facing a serious problem of reduction from its instinctivetenancy in India.Before formulation of conservation strategies for geographical protection of S. asoca genotypes available in India, it is necessary to characterize them. In the current study, the RAPD markers have been utilized effectively for categorization of S. asoca collected from 15 diverse sites of India. An initial experiment on the amplification suitability of genomic DNA samples of four S. asoca was done with 35RAPD primers. Among them only twenty sixproved their efficiency in two times repeat amplification.Total 146 bands were amplified and out of these 97 bands were found to be polymorphic. The average numbers of total band was 5.61 while average numbers of polymorphic bands was 3.73. The numbers of bands produced per primer ranged from 3 (OPE-15) to 8 (RUF205). Among all studied markers the highest percentage (100%) of polymorphism was demonstrated by only one marker (OPE-06). The lowest percent of polymorphicm (20%) was demonstrated by marker RUF211. The average percentage of polymorphism was 66.44%. Cluster analysis grouped all the S. asoca genotypes under study into two groups. Grouping of genotypes according to their sites of collection demonstrates higher similarity among or between them. The results obtained in the current study may help to formulate conservation strategies for the conservation of S. asoca genotypes.

KEYWORDS: Conservation; Fingerprinting; Genotypes; Molecular Markers; Polymorphism

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