Volume 19, number 3
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Preparation of Collagen Particles from Fish Waste Infused with Cassia auriculata

A. Nirmala* and Karthika M

Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation, Paiyanoor, Chengalpatt (Dt), Tamilnadu Pin-603 104. India.

Corresponding Author E-mail: drnirmala81@gamil.com

DOI : http://dx.doi.org/10.13005/bbra/3014

ABSTRACT: The human skin encompasses a characteristic capacity to advance the self-regeneration after harm; this capacity can be compromised beneath particular conditions, like extensive skin loss, constant wounds, deep burns, non-healing ulcers and diabetes. Improper healing can lead the wound to enter in a constant state, which increments the chance of disease to chronic state; it affects the patient health and his/her quality of life. Due to growing concerns about unhealthy consequences of chemicals in the health care system, the interest towards natural and herbal substances has been growing every day. The plant Cassia auriculata is widely used medicinal plant in India and also popular in Indigenous system of medicines like Ayurveda and siddha. The collagen was successfully extracted from the Cattla Cattla fish scales using acid solubilizing collagen extraction method and the collagen was infused with plant extract of Cassia auriculata. The characterisation such as protein estimation (Lowry method), solubility (pH 1, 3, 5, 7, 9, 11 and 13), UV visible and FTIR was performed for collagen and phytochemical analysis (Alkaloids, Flavonoids, Saponins, Tannins, Phenol, Terpenoids, Glycosides and Steroids) anti-bacterial (E. coli and S. aureus) and anti-inflammatory test in different concentration (25mg, 50mg, 100mg / ml) was performed for the plant extracts and collagen infused with plant extracts. The result of the present study was confirmed that final product prepared using collagen with plant extract can be used to prepare the dressing material such as gels, ointments, bio-films, powdered flakes etc.

KEYWORDS: Collagen; Cassia auriculata; Diabetic Foot Ulcer; Marine Source; Phytochemicals

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Nirmala A, Karthika M. Preparation of Collagen Particles from Fish Waste Infused with Cassia auriculata. Biosci Biotech Res Asia 2022;19(3).
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Nirmala A, Karthika M. Preparation of Collagen Particles from Fish Waste Infused with Cassia auriculata. Biosci Biotech Res Asia 2022;19(3). Available from: https://bit.ly/3UeQE3T

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