Volume 19, number 4
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Toxicity Study of Chlorzoxazone and Isosorbide Dinitrate using Chick Embryo

Anjali B.Tajanpure*, Neelam L. Dashputre, Pavan B. Udavant, Smita P. Kakad, Varsha S.Sandhan and Pranali P. Deshmukh

Department of Pharmacology, MET’s Institute of Pharmacy, Bhujbal knowledge City, Adgaon, Nashik-422003.

Corresponding Author E-mail: anjalitajanpure88@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3052

ABSTRACT: Background and Objectives: Number of potential drugs are underutilized due to a lack of availability of teratological data. Isosorbide Dinitrate is a saviour drug in angina prophylaxis while chlorzoxazone is a skeletal muscle relaxant and there is no adequate teratogenic study performed till date. This study aims to assess the teratological effect of these drugs on vital organs using the chick embryo model. Material and Methods: White Leghorn’s (Gallus gallus domesticus) fertilised chicken eggs were acquired from shivneri agro and hatcheries Nashik and divided into five groups (n=10) as Control, non-teratogenic, teratogenic, chlorzoxazone, and Isosorbide Dinitrate. The drug was injected via yolk inoculation and after inoculation; the eggs were re-incubated at 37.5-37.8°C and 50-60% RH for 21 days. Then the embryos were harvested and evaluated for morphological and histopathological changes. Results: The gross macroscopic examination of Isosorbide Dinitrate and chlorzoxazone treated chicks were normal. The development of the embryo was found shunted in Isosorbide Dinitrate treated group. Microscopic abrasions found in Isosorbide Dinitrate treated group are myocardial congestion, hemorrhage, hydropic degeneration, dislocation of the nucleus, splitting of cells, and infiltration of cells at all three doses. No teratogenic response was observed in chlorzoxazone treated group hence found to be safe. Conclusion: Teratogenic effect of Chlorzoxazone and isosorbide dinitrate in chick embryo provided notable details. Chlorzoxazone was found to be safe in chick embryos in the developmental phase, While Isosorbide dinitrate at highest dose was found toxic and so, it is inadvisable for its utilization in pregnancy.

KEYWORDS: Chick embryo; chlorzoxazone; Hepatotoxicity; Isosorbide dinitrate; Teratogenicity

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Tajanpure A. B, Dashputre N. L, Udavant P. B, Kakad S. P, Sandhan V. S, Deshmukh P. P. Toxicity Study of Chlorzoxazone and Isosorbide Dinitrate using Chick Embryo. Biosci Biotech Res Asia 2022;19(4).
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Tajanpure A. B, Dashputre N. L, Udavant P. B, Kakad S. P, Sandhan V. S, Deshmukh P. P. Toxicity Study of Chlorzoxazone and Isosorbide Dinitrate using Chick Embryo. Biosci Biotech Res Asia 2022;19(4). Available from: https://bit.ly/3YfHC8L

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