Optimization of the critical medium components for the production of lipase by Yarrowia lipolytica NCIM 3589 using Doehlert experimental design

I. Sarat Babu¹*, Ch. I. Raju² and G.H. Rao²

¹Department of Biotechnology, A.N.I.T.S, Sangivalasa, Bheemunipatnam, Visakhapatnam - 531 162 (India). ²Center for Biotechnology, Department of chemical engineering, Andhra University, Visakahapatnam - 530 003 (India)

ABSTRACT: Lipase production using Yarrrowia lipolytica NCIM 3589 was maximized in stationary batch culture. Response surface methodology with Doehlert experimental design was adopted to evaluate the effect on the lipase activity released in the culture medium of three most important factors, such as nitrogen concentration (1 to 3 (g/l)), carbon concentration (7.5 to 22.5 (g/l)), and salt solution concentration (5 to 15 (%v/v)). The optimal set of conditions for high lipase production were as follows: nitrogen concentration 2 g/l, carbon concentration 15 g/l and salt solution concentration 10 (%v/v). The predicated value was 9.23 U/ml and the actual value was 9.30 U/ml lipase activity.

KEYWORDS: Lipase; response surface methodology; optimization

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