Real-time PCR Analysis of ScMYB Gene Expression in Saccharum officinarum Co86032 under Drought-induced Abiotic Stress Conditions

Babu M and Geethalakshmi Sundararaman*

Department of Biotechnology, Sree Narayana Guru College, Coimbatore, Tamil Nadu, India.

Corresponding Author E-mail:s.geethalakshmi@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3153

ABSTRACT: The current research endeavor involved a meticulous analysis of the expression of MYB genes in the Saccharum officinarum Co86032 cultivar under abiotic stress conditions. The study utilized tailored primers designed to target the ScMYB protein mRNA expressed during abiotic stress like drought, which enabled successful amplification of the ScMYB60 gene in Saccharum officinarum Co86032. To quantify gene expression levels in both leaf and stem tissues, real-time PCR analysis was employed, and the specificity and accuracy of the PCR reaction were ensured through melting temperature analysis. The outcome of this study shows that the specified MYB gene got expressed even on the 18^th day of the stress which is a significant advancement in comprehending the role of MYB transcription factors in sugarcane in tolerating drought condition, and its findings may have far-reaching implications in improving sugarcane growth and development and augmenting its resilience to environmental stressors. Future investigations could potentially involve in-depth inquiries into MYB genes in sugarcane and other crops, using a diverse range of methodologies to characterize their function and regulation, with the goal of creating more robust and adaptable crops that can effectively withstand shifting environmental conditions.

KEYWORDS: CT value; FP and RP (forward and reverse primer); PCR; RTPCR; Tm value

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