Exploring Acute and Sub-Acute Toxicity of Gramine Bioactive Molecule in Wistar Rats.

Rahul R. Sable^1*, Ghanshyam B. Jadhav² and Pavan B. Udavant³

¹Department of Pharmacology, MVP’s College of Pharmacy, Nashik, Affiliated to Savitribai Phule Pune University, Maharashtra, India.

²Department of Pharmacology, MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik, Maharashtra, India.

Corresponding Author E-mail: rahulsable2812@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3180

ABSTRACT: The study evaluated the toxicity of Gramine in Wistar rats using a specified dose for acute and subacute toxicity evaluation. Female rats were given the Single dose as specified in OECD Guideline 425. Subacute toxicity was assessed using doses of 13, 27.5, and 55 mg/kg p.o. compared to a control group. After treatment, the satellite group continued operating for 14 days to assess the recovery effects. No mortality was observed at doses of 175mg/kg, but mortality was observed at doses of 550 mg/kg. In a subacute toxicity investigation, rats given Gramine orally for 28 days showed significant weight loss, reduced food intake, and normal water consumption. No harmful effects were observed for haematological or biochemical parameters, and no evidence of deterioration was found in brain, kidney, or liver histopathological examinations. The results indicate that 13, 27.5, and 55 mg/kg were considered safe doses for both short- and long-term oral therapy.

KEYWORDS: Acute; Gramine; Main Test; Subacute

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