Direct Shoot Regeneration of Plumbago zeylanica Linn. through Tissue Culture Technology

Leo Arockia Raj S, Ravindhran. R^* , Charles P, Dennis Sand Antoine Lebel

T.A.L. Samy Unit: Plant Tissue Culture and Molecular Biology, Department of Plant Biology and Biotechnology, Loyola College (Autonomous), Chennai, Tamil Nadu, India.

Corresponding Author E-mail: raviloyola1998@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3175

ABSTRACT: Chitrak is scientifically known as Plumbago zeylanica Linn. belongs to Plumbaginaceae. Root shows medicinally valuable drug in pharmacological enterprise as the humanity is certainly prone to numerous contagious ailments due to ecological imbalance triggered by the current technology development. One of its remarkable phytoconstituents is plumbagin, a naphthoquinone molecule used to treat skin conditions, tumours, and tenacious chronic rheumatoid arthritis. The objective was to standardize the procedure of Plumbago zeylanica direct-organogenesis micropropagation. The solution contained various concentrations and combinations of auxin and cytokinins, and the nodal explant was used for quick micropropagation. The maximum multiple shoots (18.24 ± 0.51) per explant with shoot length 4. 2cm ± 0.20 cm was produced as the MS medium supplemented with B5 vitamins together with 2mg/l 6- Benzyleaminopurine (BAP) and 1mg/l Indole-3- acetic acid (IAA). The regenerated shoots were rooted in half strength MS medium with B₅ vitamins that contained indole-3-butyric acid (IBA). The highest rooting frequency (100%) was seen in the 1.5 mg/l IBA-containing rooting media. The extreme roots were 9.2±0.14 per explant. The average root length was 4.2± 0.10 cm.  The in vitro rooted Shoots were then transplanted in the field showing a 100 percentage survival rate.

KEYWORDS: Direct Shoot Regeneration; Nodal segment; Plumbago Zeylanica; Plumbagin; Shoot multiplication

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