Association of Alu APO Gene Marker with Type 2 Diabetic patients of Dimapur District, Nagaland India

Semsadangla Ao¹, Moamenla Singson² and Jeyaparvathi Somasundaram^1*

¹Department of Zoology, St Joseph University, Chumoukedima, Nagaland, India.

²Eden Medical Centre, Dimapur, Nagaland, India.

Corresponding Author E-mail:jeya5001@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3193

ABSTRACT: With Type 2 Diabetes, the body either produces insufficient insulin or rejects it, which leads to an accumulation of glucose in the blood. Type 2 diabetes affects 90% of the population.. Examining the relationship between Type 2 diabetes patients from the tribal tribes of Dimapur District and the Alu APO gene marker was the main goal of this study. Using the use of random sampling, blood samples were taken from 31 Type 2 Diabetes Mellitus  patients at Eden Medical Centre in Dimapur and 31 control samples were collected from healthy individuals .  Depending on the patient availability, blood samples were taken from both male and female patients. Within a day of collecting the blood, DNA was extracted. PCR and an allele-specific marker (APO) were used for genotyping. After genotyping, results were recorded by visualizing the PCR products in UV transilluminator. With this investigation, we discovered that the female exhibits a high statistical significance in the DD genotype (0.00*). On the other hand, neither genotype is statistically significant in males.

KEYWORDS: APO gene; Dimapur; Insulin; PCR; Type 2 Diabetes

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