In Vitro Organogenesis and Epiphyllous Secondary Buds Formation in Kalanchoe tubiflora (Harvey), Hamet, (Crassulaceae)

Sathya C^1* and Abubacker M. N²

¹Department of Botany, Bishop Heber College, Tiruchirapalli, Tamil Nadu, India.

²Department of Biotechnology, National College, Tiruchirapalli, Tamil Nadu, India.

Corresponding Author E-mail: sathyasana22@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3188

ABSTRACT: In-vitro organogenesis and epiphyllous secondary buds production in Kalanchoe tubiflora were carried out using MS medium supplemented with appropriate growth regulators. This protocol can be valuable for the mass propagation of this plant species, which is crucial for effective conservation efforts. The highest yield of whitish green massive callus was obtained in MS medium supplemented with 2.0 mg/l of 2,4-dichlorophenoxy acetic acid and 1.0 mg/l of Indole-3-butyric acid. Rapid shoot formation was achieved in MS medium supplemented with 2.0 mg/l of Benzyl amino purine, 1.0 mg/l of kinetin, and 1.0 mg/l of IBA.  Effective root induction was observed in MS medium supplemented with 1.5 mg/l of Indole-3-acetic acid, 1.0 mg/l of BA, and 0.5 mg/l of Naphthalene acetic acid. Epiphyllous secondary bud induction was successfully achieved in Murashige and Skoog medium supplemented with 1.0 mg/l of BAP, 0.5 mg/l of kinetin, 0.5 mg/l of IBA, and 0.5 mg/l of Gibberellic acid 3.  The plantlets were successfully acclimatized and thrived well in the greenhouse environment. This protocol has significant potential for large-scale production of K. tubiflora and can contribute to its conservation efforts.

KEYWORDS: Callus; Epiphyllous secondary buds; Kalanchoe spp ; Organogenesis

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