Antioxidant and Anticancer Activity Assessment of N. Oleander on the Hacat Skin Cancer Cell

Manish Kumar Yadav^1* , Komal Sharma¹ and Ajay Kumar Shukla²

¹Faculty of Pharmacy, Bhupal Nobles' University, Udaipur, Rajasthan, India.

²Institute of Pharmacy, Dr Rammanohar Lohia Avadh University, Ayodhya, India.

Corresponding Author E-mail:manishky81@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3289

ABSTRACT: Aim and objective: The study aimed to use in-vitro DPPH and the HaCaT skin cancer cell line method to examine hydroalcoholic leaf extract's antioxidant and anticancer properties from Nerium oleander. Methodology: The hydroalcoholic extract of Nerium oleander was prepared to assess antioxidants and anti-cancers using the in-vitro DPPH and MTT assay method against the HaCaT skin cancer cell line. Results: The antioxidant activity of Nerium oleander hydroalcoholic leaves extract was found to be IC50 =896.9µg/ml, equivalent to IC50= 10.93µg/ml of ascorbic acid, and IC50 = 91.49±0.181µg/ml effective against the HaCaT skin cancer cell line. Further research against carcinogenesis from the hydroalcoholic extract of Nerium oleander leaves, which demonstrated therapeutic potential against cancer cells, can yield significant results. Conclusion: The bioactive chemicals of Nerium oleander leaves may be beneficial for treating skin cancer. Limitations: The study's emphasis on in-vitro tests, which might not accurately capture the nuances of in-vivo settings, has limitations. To confirm the extract's potential for medicinal use, more research should examine its safety and efficacy in animal models and human trials.

KEYWORDS: Hydroalcoholic extract; HaCaT skin cancer cell line; N. oleander; Skin cancer

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