Characterization and Genetic analysis of Actinomycetes from Mangrove and Coastal Environments: Enzyme Production, Dye Degradation and Antibiotic Resistance

P. Vanathi^1*, Uma Sundaresan² and Kavitha Manivannan³

¹Department of Microbiology, Bharathidasan Arts and Science College, Ellispettai, Erode, TamilNadu,India.

²Department of Food Process Engineering, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India.

³Department of Microbiology, Tharb Camel Hospital, Qatar.

Corresponding Author E-mail:vanathisekar3@gmail.com

ABSTRACT: Actinomycetes from mangrove and coastal environments were studied for their potential in biotechnology and environmental management. This research aimed to isolate and characterize these microbes, focusing on enzyme production, dye degradation, and antibiotic resistance. Samples were collected from various mangrove and coastal sites. Actinomycetes were isolated using selective media and identified through morphological and biochemical tests. Genetic characterization was performed using 16S rRNA sequencing. Enzyme production was evaluated through specific proteases, lipases, and cellulase assays. Dye degradation studies involved incubating actinomycetes with synthetic dyes and measuring degradation efficiency using spectrophotometric methods. Antibiotic resistance was assessed using disk diffusion and minimum inhibitory concentration (MIC) test. The study focused on isolating a variety of Actinomycetes from mangrove and coastal environments, assessing their potential for enzyme production and dye degradation.

KEYWORDS: Actinomycetes; Calcium carbonate; Dye degradation; 16SR RNA; SDS-PAGE

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