Investigation on Biocontrol Efficacy of Fungal Metabolites Produced by Alternaria alternata Against Invasive Parthenium weed

Komal Sahu¹, Vikas Kumar² , Galymbek Seralin³, Urvasha Patyal¹, Anuj Sahu⁴, Anjali Kanwal¹and Raj Singh^1*

¹Department of Bio-Sciences and Technology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala (Haryana), India.

²Department of Microbiology, International Medical School, Kenzhegali Sagadiyev, University of International Business, Almaty, Kazakhastan

³Department of Pre-clinical, International Medical School, Kenzhegali Sagadiyev, University of International Business, Almaty, Kazakhstan

⁴School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Khordha, Odisha, India.

Corresponding Author E-mail:dr.rajsingh09@gmail.com

ABSTRACT: One of the most aggressive invasive weeds, Parthenium hysterophorus (Asteraceae) is generating serious difficulties for the environment, economy, and livestock in India and other parts of the world. The present study was conducted to isolate and identify the fungal pathogens from the leaves of Parthenium weed and evaluate their cultural filtrates against the growth of the weed. Alternaria alternata was isolated from the infected Parthenium leaves by using standard isolation techniques utilizing potato dextrose agar (PDA), during an extensive search for natural enemies of P. hysterophorus. After applying Koch's postulates and leaf bioassay, it was discovered that A. alternata was found pathogenic and can be used as biocontrol agent of the weed. Seed germination bioassay was performed to check the herbicidal potential of A. alternata metabolites against Parthenium plant. The fungal pathogen's metabolites caused severe harm to the leaves and seeds of P. hysterophorus. This research aims to explore the biological control efficacy of isolated fungus A. alternata to inhibit Parthenium growth.

KEYWORDS: Biocontrol agent; herbicidal; invasive; livestock; Parthenium; postulates

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