In Vitro Evaluation of Biological Activities of Three Different Medicinal Plant Extracts

Kalyani Chepuri^1*, Chidepudi Devi Sri Lalitha Naga Tulasi¹, Manikantha Dunna², Chittepu Pranitha¹, Kathuroju Harikrishna¹and Vadakavila Geethikalal¹

¹Centre for Biotechnology, University College of Engineering Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad, Telangana, India

²Health Sciences Centre, Texas Tech University, Lubbock, Texas, USA

Corresponding Author E-amil:kalyanichepuri@jntuh.ac.in

ABSTRACT: This research evaluates the biological activities of three medicinal plants: Butea monosperma, Delonix regia, and Spathodea campanulata. These plants, belonging to the Fabaceae and Bignoniaceae families, were investigated for their antibacterial, antifungal, antioxidant, and cytotoxic properties. Phytochemicals were extracted from the flowers and leaves using methanol, ethanol, and acetone. The antibacterial and antifungal activities were assessed using a disc diffusion assay against pathogens S. epidermidis, B. cereus, E. coli, K. aerogenes, P. vulgaris, S. aureus, and C. albicans. The antioxidant potential was evaluated using the DPPH assay, and the cytotoxic activity was measured using the MTT assay on human lung cancer (A549) cells. Results indicated that ethanol extracts demonstrated the highest antimicrobial efficacy against both Gram-negative and Gram-positive bacteria, as well as the fungal strain Candida albicans. The extracts also demonstrated antioxidant activity, with the Butea monosperma ethanol extract showing the highest activity at an IC₅₀ value of 61.55±1.22 μg/mL. Methanol extracts showed significant cytotoxic effects on the A549 cell line, with Butea monosperma having an IC₅₀ value of 36.12±1.01 μg/mL. These findings suggest that the extracts from these plants have potential therapeutic applications against bacterial and fungal infections, oxidative stress, and cancer cells.

KEYWORDS: Anticancer activity; Antimicrobial activity; Antioxidants; Butea monosperma; Delonix regia; Spathadea companulata

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