Isolation and Molecular Identification of Extreme an Halotolerant Staphylococcus Sp. 13cc from Brackish Water of Chilika Lake

P. D. Mahapatra and B. B. Mishra

Department of Microbiology, CPGS, OUAT, Bhubaneswar - 751 003, India.

ABSTRACT: Brackish water is the most extraordinary reservoir of bacterial community with an adaptability of tolerance to saline condition. In this research work, the halotolerance of the bacterial isolates were screened for identification and characterization of halotolerant bacteria from the brackish water of the chilika lake,Odisha. Representative samples of water was collected aseptically from four important sites of the lake viz Barkul,Kalupada Ghat,Parikuda and Rambha during the period from January to June 2012. Maximum possible number of culturable bacteria were obtained and screened for halotolerance. A total of 25 bacterial isolates were obtained and almost all the isolates survived upto 1 M NaCl but only one bacteria tolerated above 2.5M NaCl and was extremely halotolerant .This strain was identified as Staphylococcus sp.13CC. and designated with accession number KF657328 . It is a potential urease producer which is an important industrial enzyme. Further assay of enzyme is yet under study.

KEYWORDS: Halotolerant bacteria; Staphylococcus sp.; Urease

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