Preparation of Maltotriose syrup from microbial Pullulan by using Pullulanase Enzyme

Bishwambhar Mishra*, Akula Manikanta and Deveeka Zamare

Department of Biotechnology, Sreenidhi Institute of Science and Technology, Ghatkesar, Hyderabad – 501 301

DOI : http://dx.doi.org/10.13005/bbra/2058

ABSTRACT: The present work deals with the preparation and single point optimization of maltotriose syrup prepared from microbial pullulan. The enzyme pullulanase was used in this process for hydrolysis of pullulan. Production of pullulan was carried out in a shake flask fermenter with isopropanol precipitation. Various parameters like time, temperature, pH, amount of ethanol and effect of amount of enzyme on the hydrolysis of pullulan were optimized by single point optimization studies. The optimized values for the process parameters were 8h, 46°C, 5.5,  6 (v/v) and 10 ASPU/g  for time of hydrolysis, temperature, pH , amount of ethanol and amount of pullulanase enzyme respectively. The results obtained from HPLC, confirmed that major component present in the hydrolysate were composed of maltotriose.  Results of experiments indicated that this was a promising way of preparation of maltotriose.

KEYWORDS: Pullulan; Pullulanase; Maltotriose; Hydrolysis; HPLC; Optimization

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