Development of new reversed-phase HPLC method for the determination of Mangiferin in Mangifera indica Linn.

Pradeep Kumar¹*, Vishal Srivastava¹ and Lalit Kumar²

¹Department of Pharmaceutical Analysis, PES College of Pharmacy, Bangalore, Karnataka India.

²Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Karnataka India.

Corresponding Author E-mail: pradeep_alpine@yahoo.co.in

ABSTRACT: Mangiferin is the active constituent obtained from the dried parts such as leaves and barks of the Mango tree (Mangifera indica L.) which belongs to the family Anacardiaceae. Analytical method is developed and validated for the determination of Mangiferin in Mangifera indica by reversed-phase HPLC method. The HPLC method is described for determination of Mangiferin from leaves and barks Extract. The separation of the Mangiferin was achieved on a Lichrospher ® 100; RP- 18e (5 μm) column using mobile phase Acetonitrile: Buffer (Potassium dihydrogen orthophosphate (1.36 gm) in 900 ml HPLC grade water, adjust the PH to 2.5-2.8 with orthophosphoric acid and detection was carried at 254 nm (Isocratic Elution). The Lichrospher ® 100; RP-18e (5 μm) shows most favorable chromatographic parameters for analysis of Mangiferin in Mangifera indica. The linear range for Mangiferin was found to be 15.0-1000 μg mL-1. The Method has been successfully validated for linearity, Specificity, Precision, Accuracy, range and with good recoveries. Hence the present RP-HPLC method is suitable for assay of the Mangiferin in raw materials, extracts in quality control laboratories.

KEYWORDS: Mangiferin; leaves; bark; RP-HPLC; isocratic elution; alcoholic extracts

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