Spectrophotometric Methods For Determination of Gallic Acid by Oxidative Coupling With Orcinol

C. Bala Sekharan¹* and S. Vijaya Saradhi²

¹Department of Biotechnology, P.B. Siddhartha College of Arts and Science, Vijayawada - 520 010 India.

²Department of Biotechnology, Koneru Lakshmaiah College of Engineering, Vaddeswaram - 02 India.

Corresponding Author: E-Mail balumphil@gmail.com

ABSTRACT: A simple, sensitive and reproducible spectrophotometric method is developed for the determination of gallic acid . This Method is based on oxidative coupling reaction between gallic acid with orcinol in the presence of Hydrogen peroxide and enzyme horseradish peroxidase to produce colored chromogen (lmax at 620 nm). Results of analysis were validated statistically and by recovery studies. This method is successfully employed for the determination of gallic acid in oils.

KEYWORDS: Gallic acid; orcinol; Visible Spectrophotometric determination; Beer’s Law

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