Detection of Escherichia coli Pathotypes from the Cases of Diarrhea

Maryam Zarringhalam¹, Hossein Goudarzi^2*, Mohammad Reza Nahaei³, Mojgan Bandehpour⁴ and Gholamhassan Shahbazi³

¹Department of Microbiology, International Branch of Shahid Beheshti University of Medical Sciences, Tehran, Iran. ²Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ³Department of Microbiology, Tabriz University of Medical Sciences, Tabriz, Iran. ⁴Department of Biotechnology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.   Corresponding Authro Email:  medicalopto@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2028

ABSTRACT: Escherichia coli is a member of the normal microbiota,and also a common inhabitant of the human and warm-blooded animal intestinal tract, which several pathogenictypes of it can cause different diseases.One hundred and fourty seven E. coli isolates were obtained from the cases of diarrhea during 2013-2014. In order to detect E. coli pathotypes a mixture of nine primer pairs was used in three multiplex PCR assays. The PCR assays detected 26 ETEC, 20 EAEC, 11 EHEC, 9 EPEC and 2 EIEC. All E. coli strains were serogrouped by a latex agglutination test using a commercial antiserum. According to the results, 53 isolates were positive and according to kit regents, 3 different groups were identified. The findings also indicated that the E. coli strains recovered in this study expressed high levels of resistance to antimicrobials that are commonly used in clinical medicine. In conclusion,differentiation between the diarrheagenic E. coli pathotypes is very importantsince they are involved in cancer or cellular senescence. It was concluded that at attempts at economy are better conducted via finding appropriate criteria for requesting a stool culture rather than at limitation of the microbiological evaluation of specimens that are subjected.

KEYWORDS: Serogrouping; Multiplex; PCR; Detection; Escherichia coli

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