Volume 13, number 1
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Analysis of Genetic Diversity Amongst Fusarium Spp. Associated With Root Rot of Apple

Mohit Sharma, Apporva Kapatia and Saurabh Kulshrestha*

Shoolini University of Biotechnology and Management Sciences, Bajhol, Solan, Himachal Pradesh.

DOI : http://dx.doi.org/10.13005/bbra/2055

ABSTRACT: The Genus Fusariumis associated with crop diseases of horticultural and agricultural commodities and is considered to be a destructive pathogen.Geneticdiversity among ten different isolates of Fusarium species isolated from apple rhizosphere from different locations of Himachal Pradesh was analysed using Random Amplified Polymorphic DNA (RAPD-PCR). Molecular characterization using ITS sequencing revealed that out of 10isolates ofFusarium , three belongs toF.oxysporum, two belongs to F.solani,one belongs to F. equiseti and four wereFusariumspecies. Seven random primers were used for their genetic characterization using RAPD analysis. The dendrogram obtained from the UPGMA study characterized ten isolates into one main cluster and nine independent branches on the basis of similarity value 0.65.

KEYWORDS: Fusarium; Apple; RAPD; ITS; TEF

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Sharma M, Kapatia A, Kulshrestha S. Analysis of Genetic Diversity Amongst Fusarium Spp. Associated With Root Rot of Apple. Biosci Biotech Res Asia 2016;13(1)
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Sharma M, Kapatia A, Kulshrestha S. Analysis of Genetic Diversity Amongst Fusarium Spp. Associated With Root Rot of Apple. Biosci Biotech Res Asia 2016;13(1). Available from: https://www.biotech-asia.org/?p=7573

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