Evaluation of Free Radical Scavenging Potential of Ethanolic Extract of Artemisia Nilagirica (Clarke) Pamp. Leaves

V. Suseela¹*, V. K. Gopalakrishnan¹ and D. Chandrapraba²

¹Department of Biochemistry, Karpagam University, Coimbatore - 641 021 India. ²Department of Biochemistry, Sree Narayanaya Guru college, Coimbatore - 641 021 India.   Corresponding Author E-mail: suseelavivekananthan@yahoo.com

ABSTRACT: Antioxidant potential of leaves of Artemisia nilagirica was studied using different in vitro models like 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothizoline-6-sulphonate) (ABTS), nitric oxide, superoxide, hydroxyl radical and lipid peroxidation. The ethanol extract showed maximum scavenging activity (89.06%) of ABTS radical followed by the scavenging of DPPH radical (84.94%) and superoxide radical (79.69%) respectively. Total antioxidant capacity was found to be 61.3 mg ascorbic acid equivalents at 500μg/ml extract concentration. However, the extract showed only moderate lipid peroxidation inhibition activity. Total phenols and flavonoids were found to be 69.71 + 1.7 mg Gallic acid equivalents/g of dry material and 28.41 + 0.6 mg Quercetin equivalents/g of dry material respectively. These results suggest that phenolics and flavonoids in the leaves provide substantial antioxidant activity.

KEYWORDS: Artemisia nilagirica; free radical; in vitro studies

[ HTML Full Text]